Parasites

Morphology

Cytology:
Detection of characteristic Schistosoma eggs in urine or stool specimens, less commonly also in cervical smears. The eggs, measuring 110 to 170 µm × 50 to 70 µm, are oval to elongated and usually contain a mature miracidium at the time of excretion. Species assignment is based on egg morphology and the type of specimen: S. haematobium with a terminal spine, usually in urine; S. mansoni with a prominent lateral spine, usually in stool; S. japonicum with a small, often inconspicuous lateral spine.

Histology:
Usually a granulomatous inflammatory reaction to retained eggs, often with fibrosis and calcification.
In intestinal or hepatosplenic schistosomiasis, eggs are found in the bowel wall, liver, and periportal tissue, with possible ulceration, hyperplasia, polyposis, periportal fibrosis, and portal hypertension.
In urogenital schistosomiasis, eggs are found in the urinary tract and are accompanied by chronic inflammation, fibrosis, and calcification.
In histological sections, the egg shell of S. mansoni stains usually positive (red), whereas the egg shell of S. hematobium stains negative (blue) with ZN (1).

Clinical notes

Schistosomiasis (syn. bilharzia) is a parasitic intravascular infection in which chronicity and severity are largely determined by the host's immune response to the trematode worms and to egg deposition in the tissues.
Cercarial dermatitis (urticarial rash) and Katayama syndrome (systemic hypersensitivity reaction) are early manifestations. Following freshwater exposure, cercarial dermatitis may initially occur; Katayama syndrome typically develops weeks later, with fever, rash, headache, myalgia, respiratory symptoms, and eosinophilia. 
Chronic/advanced manifestations are caused by local inflammatory response to Schistosoma eggs trapped in host tissues and are species-dependent: S. mansoni and S. japonicum more often cause intestinal and hepatosplenic disease, with abdominal pain, diarrhea, constipation, and blood in the stool; severe cases may be associated with liver fibrosis, splenomegaly, and portal hypertension. Urogenital disease is characterized by dysuria and hematuria. Chronic S. haematobium infection is also associated with bladder fibrosis, renal damage, and an increased risk of bladder carcinoma. CNS manifestations due to ectopically deposited eggs are rare.

Ancillary Testing

Urine filtration, Kato-Katz technique for stool examination, serology, antigen detection, PCR, radiology (CXR, abdominal/pelvic US)

Additional Information

Infection occurs after contact with freshwater through skin penetration by cercariae; the snail serves as the intermediate host. Eggs typically appear in urine or stool 1 to 3 months after exposure.
After freshwater exposure in regions where S. mansoni and S. haematobium are co-endemic, both stool and urine samples should be examined.