Parasites

Morphology

Amastigotes are the intracellular pathogenic form of parasites, 2-4 µm in size, round-to-oval with a thin cell membrane, a large nucleus, and a rod-shaped kinetoplast, lacking an external flagellum.
Leishmania are usually found intracellularly.

Cytology:
Bone marrow or spleen aspirate with macrophages densely packed with amastigotes (Leishman-Donovan bodies) 2-4 µm; round to oval with a big, eccentrically placed nucleus and a rod-shaped kinetoplast with a dot-dash-like pattern; numerous free amastigotes in the background.

Surrounding Reaction:
Abundant macrophages, granulocytes, and lymphoplasmacytic infiltrate.
Reactive, physiologically occurring cells.

Histology:
Bone marrow core biopsy hypercellular with marked macrophage expansion and numerous intramacrophage amastigotes on Giemsa or HE.
Liver with sinusoidal and portal macrophage infiltration (Kupffer cell hyperplasia).
Spleen with white-pulp depletion and diffuse macrophage/plasma cell infiltrates.
Lymph nodes with paracortical histiocytosis. Amastigotes intramacrophagic; granulomas may be present; necrosis is uncommon. Amastigotes are GMS-negative.

Differential Diagnosis:

• Trypanosoma cruzi - both amastigotes are indistinguishable; confirmation by serology or PCR is necessary
• Histoplasmosis - amastigotes are GMS-negative
• Malakoplakia

Clinical notes

Patients show 5 main symptoms: Hepatosplenomegaly, pancytopenia, lymphadenopathy, fever, and, in the advanced stages, cachexia - fatal if untreated.
Chronic cough indicates the rare involvement of the lungs. Imaging studies reveal, in addition to massive hepatosplenomegaly, diffuse interstitial consolidations in the lungs. 

Ancillary Testing

PCR/RT-PCR on bone marrow, blood (EDTA), or tissue; Antigen/Serology: rK39 rapid test (good rule-in in endemic settings), rK28/ELISA or DAT (direct agglutination test) as alternatives; Montenegro skin test (Leishmanin test)

Additional Information

Leishmania spp. are hemoflagellates and have two morphological stages: amastigotes (human tissue) and promastigotes (within vector). 
Infection with certain Leishmania spp. can cause cutaneous, mucocutaneous, and visceral leishmaniasis.
The subspecies within the L. donovani complex cause visceral disease (syn. kala-azar): L. donovani, L. chagasi, and L. infantum.
Visceral leishmaniasis occurs endemically in tropical and subtropical regions, and rarely in the Mediterranean area. It predominantly affects children and young adults.
The pathogens, transmitted percutaneously by sandflies, multiply within macrophages. Through the bloodstream, the pathogens spread to the liver, spleen, bone marrow, and lymph nodes. In severe infections, all organs may be affected.
The incubation period is 3 to 8 months.